General Cell Based Assays
Eurofins Discoverx PathHunter eXpress PROKR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0282E2CP2M)
The PathHunter PROKR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PROKR2 and EA-tagged β-Arrestin-2. Activation of PROKR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Mouse TNF- ASY
The Meso Scale Discovery U-PLEX Mouse TNF-alpha Assay is a multiplex immunoassay kit that enables simultaneous measurement of mouse tumor necrosis factor alpha (TNF-alpha) in serum plasma and cell culture supernatants It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This assay requires 50 L or less sample volume and can be fully automated on the MSD instrument platforms The lower limit of detection is 0 04 pg/mL
Medchemexpress LLC cGAS Human His-SUM 10ug
Recombinant human cyclic GMP-AMP synthase (cGAS) fragment G161-F522, expressed in E. coli and supplied lyophilized with an N-terminal 6xHis-SUMO tag. The protein is purified to >90% and provided with storage and reconstitution guidance to preserve activity for biochemical and structural applications.
- Recombinant human cGAS fragment G161-F522 with N-terminal His-SUMO tag.
- Expressed in E. coli and purified to greater than 90% purity.
- Lyophilized formulation for improved stability and convenient storage.
- Buffer contains trehalose to protect protein during lyophilization.
- Suitable for biochemical assays, enzymatic activity, and structural studies.
- Includes storage and reconstitution guidance to maintain activity.
Abcam Isocitrate Dehydrogenase Assay Kit (Colorimetric)
Isocitrate Dehydrogenase Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress mCCR7 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0528PE2MCP0L)
The PathHunter mCCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19/MIP-3β) based activation of mCCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR7 and EA-tagged β-Arrestin-2. Activation of mCCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems QuantiFluo™ Ammonia Assay Kit. For quantitative determination of ammonia/ammonium concentration by fluorimetry at 360nm/450nm (compatible on handheld fluorimeter catalog# FL360450).
For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism. Key Features: Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450nm. Samples: Biological (eg urine) and environ-mental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 200 tests. Detection limit: 12 µM.
AURORA BIOLABS LLC PKMYT1 Binding Assay, 384 rxn
A fluorescence-labelled tracer, that can bind to PKMYT1, and the N-terminal GST-tagged full-length human PKMYT1 are used in this assay kit. A Terbium-labeled anti-GST antibody binding to the GST-PKMYT1 serves as a fluorescence donor (HTRF donor). If the fluorescence-labeled tracer binds to the PKMYT1, the binding brings Terbium on the anti-GST antibody close to the fluorophore on the tracer (HTRF acceptor). Activation of the Terbium results in fluorescence resonance energy transfer (FRET). Thus, the binding status can be quantitively measured by calculating the ratio of the emission fluorescence intensity of the acceptor (665 nm) and donor (620nm). The competitive binding of a non-fluorescence-labeled compound will reduce the FRET signal.
American Research Products Inc Human EXtracellular superoXide
Human EXtracellular superoXide dismutase [Cu-Zn] (SOD3) ELISA kit from CUSABIO Detection Range 7 8 pg/mL-500 pg/mL Sensitivity 1 95 pg/mL Sample serum plasma cell culture supernates tissue homogenates saliva urine Method Sandwich
Eurofins Discoverx PathHunter Human GPR20 β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human GPR20 β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure GPR20 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human Syk Kinase Activity Cell-Based Assay Kit (U2OS)
The PathHunter Human Syk Stable Cell Line Kinase Activity Assay (U2OS) measures SYK CTK activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human TrkC-P75 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human TrkC-P75 Stable Cell Line Functional Kinase Assay (U2OS) measures TrkC-P75 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Mouse EBI2 (GPR183) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse EBI2 (GPR183) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure mEBI2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
CPC Scientific H-Thr-Ser-Gly-Pro-Leu-Gly-Asn-Leu-Ala-Glu-Glu-Leu-Asn-Gly-Tyr-Ser-Arg-Lys-Lys-Gly-Gly-Phe-Ser-Phe-Arg-Phe-NH2 (trifluoroacetate salt) 1MG
An RF-amide peptide ligand for SP9155, an orphan G-protein-coupled receptor with high homology to orexin, neuropeptide FF and CCK receptors. It has been shown to potently activate SP9155 with an EC50 of 7 nM.ONE-LETTER SEQUENCE: TSGPLGNLAEELNGYSRKKGGFSFRF-NH2MOLECULAR FORMULA: C127H195N37O37MOLECULAR WEIGHT:2832.17STORAGE CONDITIONS: -20 5C, CAS REGISTRY NUMBER: [600171-68-4], RESEARCH AREA: PainREFERENCES: N.Chartrel et al., Proc. Natl. Acad. Sci. USA, 100, 15247 (2003); Y.Jiang et al., J. Biol. Chem., 278, 27652 (2003)
Eurofins Discoverx PathHunter Human CRTH2 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human CRTH2 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CRTH2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.